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atcc crl 1964 cells  (ATCC)


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    ATCC atcc crl 1964 cells
    Atcc Crl 1964 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+crl+1964+cells/Clone+15+HL-60%3B+Promyelocytic+Leukemia%3B+Human/pmc12906310-257-17-22
    Average 90 stars, based on 7 article reviews
    atcc crl 1964 cells - by Bioz Stars, 2026-10
    90/100 stars

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    Transfection:

    Article Title: Mendelian randomization analysis identifies HLA‐A and AP2M1 as genetic biomarkers linked to immune–endocytic crosstalk in intervertebral disc degeneration
    Article Snippet: For immune cell infiltration analysis, Transwell chambers (Corning) were used. .. After transfection, NP cells were seeded in the upper chamber, and THP‐1 and HL‐60 clone 15 (HC15; ATCC‐CRL‐1964) cells (both obtained from ATCC) were co‐cultured in the lower chamber. .. Before co‐culture, HC15 cells were differentiated for 5 days using 0.5 mM SB (sc‐202341, Santa Cruz Biotechnology) and 10 ng/mL IL‐5 (NBP2‐34897, Biotechne GmbH, Wiesbaden Nordenstadt).



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    Experimental scheme showing the workflow of studying the eosinophilic cell line HC15. The cell line cells were cultured at pH 7.6-7.8. For differentiation, they were incubated with 0.5 mM SB for 5 days with or without the addition of 10 ng/mL IL-5. The cells were compared to primary Eos and Neutros in a comprehensive proteomics approach. Subsequently, the cell line cells were characterized using different techniques such as RT-qPCR, ELISA and staining methods paired with microscopy. Lastly, their similarity to Eos was tested in functional assays such as migration, aggregation, adherence and activation assays.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: Experimental scheme showing the workflow of studying the eosinophilic cell line HC15. The cell line cells were cultured at pH 7.6-7.8. For differentiation, they were incubated with 0.5 mM SB for 5 days with or without the addition of 10 ng/mL IL-5. The cells were compared to primary Eos and Neutros in a comprehensive proteomics approach. Subsequently, the cell line cells were characterized using different techniques such as RT-qPCR, ELISA and staining methods paired with microscopy. Lastly, their similarity to Eos was tested in functional assays such as migration, aggregation, adherence and activation assays.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Cell Culture, Incubation, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Staining, Microscopy, Functional Assay, Migration, Activation Assay

    The proteome of IL-5-differentiated cells resembles eosinophils in differentiation, chemotaxis, and migration pathways, but not in immunity and activation pathways. The proteomes of HC15 (N=4), DHC15 (N=4), IHC15 (N=4), Eos (N=6) and Neutros (N=6) were measured using a shotgun proteomics approach. (A) PCA comparing all measured groups produced using the Perseus software version 4.1.3.0. (B, C) Volcano blot of DAPs in (B) Eos vs. IHC15 or (C) Neutros vs. IHC15. The data were plotted using R version 4.0.3. DAPs were considered significant with an FDR <0.05 and a log 2 FC of >1. (D) GSVA of DAPs in IHC15 vs. Eos. The used gene sets were downloaded from the Molecular signatures Database. (E, F) Venn diagrams of gene IDs found in (E) HC15 vs. IHC15 vs. Eos or in (F) HC15 vs. DHC15 vs. Eos. The graphs were produced using R version 4.3.3. (G, H) Pathway enrichment analysis of exclusively shared proteins between (G) IHC15 and Eos or (H) DHC15 and Eos computed using Metascape . The data were analyzed and visualized using R version 4.0.3.-4.3.3.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: The proteome of IL-5-differentiated cells resembles eosinophils in differentiation, chemotaxis, and migration pathways, but not in immunity and activation pathways. The proteomes of HC15 (N=4), DHC15 (N=4), IHC15 (N=4), Eos (N=6) and Neutros (N=6) were measured using a shotgun proteomics approach. (A) PCA comparing all measured groups produced using the Perseus software version 4.1.3.0. (B, C) Volcano blot of DAPs in (B) Eos vs. IHC15 or (C) Neutros vs. IHC15. The data were plotted using R version 4.0.3. DAPs were considered significant with an FDR <0.05 and a log 2 FC of >1. (D) GSVA of DAPs in IHC15 vs. Eos. The used gene sets were downloaded from the Molecular signatures Database. (E, F) Venn diagrams of gene IDs found in (E) HC15 vs. IHC15 vs. Eos or in (F) HC15 vs. DHC15 vs. Eos. The graphs were produced using R version 4.3.3. (G, H) Pathway enrichment analysis of exclusively shared proteins between (G) IHC15 and Eos or (H) DHC15 and Eos computed using Metascape . The data were analyzed and visualized using R version 4.0.3.-4.3.3.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Chemotaxis Assay, Migration, Activation Assay, Produced, Software

    Morphology and surface marker expression of cell line cells and Eos. (A) Hemacolor ® staining of cell line cells and leukocytes. Black arrows indicate unregular nucleus structure and red arrows indicate high granular content in the differentiated cell line cells. White arrows point to Eos in the leukocytes. Representative images of 5 independent experiments. Scale bar depicts 50 µm. (B-D) mRNA expression levels of surface markers relevant in eosinophil biology: (B) IL5RA , (C) ADGRE1 , (D) SIGLEC8 measured in RT-qPCR (N=5-7). (E) Surface exposure of CCR3 measured by flow cytometry (N=8-9). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, ***P<0.001; #P<0.05, ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: Morphology and surface marker expression of cell line cells and Eos. (A) Hemacolor ® staining of cell line cells and leukocytes. Black arrows indicate unregular nucleus structure and red arrows indicate high granular content in the differentiated cell line cells. White arrows point to Eos in the leukocytes. Representative images of 5 independent experiments. Scale bar depicts 50 µm. (B-D) mRNA expression levels of surface markers relevant in eosinophil biology: (B) IL5RA , (C) ADGRE1 , (D) SIGLEC8 measured in RT-qPCR (N=5-7). (E) Surface exposure of CCR3 measured by flow cytometry (N=8-9). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, ***P<0.001; #P<0.05, ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Marker, Expressing, Staining, Quantitative RT-PCR, Flow Cytometry

    TF profile of the cell line cells and Eos. (A-C) mRNA expression levels of TFs relevant to eosinophil differentiation: (A) SPI1 , (B) GATA1 and (C) ID2 (N=5-7). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01; ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: TF profile of the cell line cells and Eos. (A-C) mRNA expression levels of TFs relevant to eosinophil differentiation: (A) SPI1 , (B) GATA1 and (C) ID2 (N=5-7). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01; ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Expressing

    Differentiation induces granule protein expression in HC15 cells on a mRNA level. (A, B) mRNA expression levels of eosinophil-specific granule proteins: (A) PRG2 and (B) EPX (N=5-7). (C-E) Granule protein levels. (C) Granule proteins are stained in red (EMBP) and green (EPX), respectively. DAPI (blue) and wheat germ agglutinin (yellow) were used to identify cell nuclei and cell borders, respectively. Representative images of 6 independent experiments. Scale bar depicts 50 µm. (D, E) Quantification of granule protein levels (D) EMBP and (E) EPX. 5 pictures of randomly chosen areas were analyzed. The mean fluorescence intensity per cell was determined using ImageJ. Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01; #P<0.05; Kruskal-Wallis test with Dunn’s multiple comparisons. MFI, mean fluorescent intensity.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: Differentiation induces granule protein expression in HC15 cells on a mRNA level. (A, B) mRNA expression levels of eosinophil-specific granule proteins: (A) PRG2 and (B) EPX (N=5-7). (C-E) Granule protein levels. (C) Granule proteins are stained in red (EMBP) and green (EPX), respectively. DAPI (blue) and wheat germ agglutinin (yellow) were used to identify cell nuclei and cell borders, respectively. Representative images of 6 independent experiments. Scale bar depicts 50 µm. (D, E) Quantification of granule protein levels (D) EMBP and (E) EPX. 5 pictures of randomly chosen areas were analyzed. The mean fluorescence intensity per cell was determined using ImageJ. Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01; #P<0.05; Kruskal-Wallis test with Dunn’s multiple comparisons. MFI, mean fluorescent intensity.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Expressing, Staining, Fluorescence

    mRNA levels of various adhesion markers in cell line cells and Eos. mRNA expression levels of relevant adhesion markers: (A) SELPLG , (B) SLC44A2 , (C) CD63 , (D) ITGAM , (E) CD40 , (F) ITGAL , (G) TREM1 ) (N=5-7). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01, ***P<0.01; #P<0.05; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: mRNA levels of various adhesion markers in cell line cells and Eos. mRNA expression levels of relevant adhesion markers: (A) SELPLG , (B) SLC44A2 , (C) CD63 , (D) ITGAM , (E) CD40 , (F) ITGAL , (G) TREM1 ) (N=5-7). Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01, ***P<0.01; #P<0.05; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Expressing

    IL-5-differentiated cells produce a granulocyte- and T-cell-attracting inflammatory milieu. Cytokine- and granule protein-secretion profiles from unstimulated cell line cells and Eos measured by ELISA (N=5-8): (A) CCL-5, (B) EPX, (C) IL-8. Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01, ***P<0.01; ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Journal: Frontiers in Immunology

    Article Title: The HL-60 clone 15 cell line as a model for leukocyte migration–possibilities and limitations

    doi: 10.3389/fimmu.2025.1515993

    Figure Lengend Snippet: IL-5-differentiated cells produce a granulocyte- and T-cell-attracting inflammatory milieu. Cytokine- and granule protein-secretion profiles from unstimulated cell line cells and Eos measured by ELISA (N=5-8): (A) CCL-5, (B) EPX, (C) IL-8. Data is depicted as boxplots. * shows significance vs. Eos, # shows significance vs. HC15. *P<0.05, **P<0.01, ***P<0.01; ##P<0.01; Kruskal-Wallis test with Dunn’s multiple comparisons.

    Article Snippet: HC15 cells (ATCC-CRL-1964) were purchased from LGC Standards GmbH (Teddington, U.K.).

    Techniques: Enzyme-linked Immunosorbent Assay